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How to Use Proteases to Purposefully Digest Proteins

In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified,...
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Article

Reframing – A Way to Cope With Stress in Graduate School

I’m an anxiety-ridden stress ball 90% of the time. Graduate school only amplifies my nervous energy, and it’s a struggle. However, recently, while I rushed to catch a bus, I had a life altering experience...
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Article

Open and Closed: Two Ways to Grow Your Own Algae

In my last article, I talked about the basic protocols and experiments conducted in the process of converting algae into biofuel. Our ability to culture algae has efficiently improved over the years. Continous...
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Article

How to Improve Plasmid Yield Using Antibiotics

After the initial excitement of growing and isolating plasmid DNA, routinely preparing plasmid mini/midi/maxi preps gets boring. You definitely want a way to squeeze the maximum amount of plasmid DNA out of...
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Article

Fine-Tune Your MALDI-TOF to Produce Good-looking Mass Spectra

Mass Spec is all about getting the perfect peaks. Without a good peak assigning the correct mass is impossible and you cannot make accurate identifications. Make sure you know how to adjust your MALDI-TOF...
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Article

Science Research at the Crossroads: Academia versus Industry

Academia or industry? Basic research or applied research? You are thinking of what to do next. What is right for you? Honestly, it is a never ending discussion. So what should you do? Here are some insider...
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Article

Methods for Relative Quantification of qPCR Data. Yes, There is More Than One.

As all of you probably know, methods for calculating relative gene expression from qPCR data include: a) double delta Ct (ΔΔCt) and b) that one other method. Chances are you’ve probably gotten...
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Article

A Quick Guide to Screening by Restriction Digestion

PCR screening is great for identifying potential positive clones after cloning/subcloning. However, you’ll still want to confirm that the DNA sequence is correct for any positives identified. The easiest and...
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Article

How to Destroy your Flow Cytometry Data in 3 Easy Steps: Snap, Crackle, and Pop

While many scientists are methodical and precise, some of us like to live on the edge. Read a protocol all the way through? No thanks, I’ll take my chances and guess what concentration of HCl I should use....
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Article

How to Effectively Organize a Research Lab

An academic lab is a unique working environment. Lab members are expected to take responsibility for their own research projects and perform the work quickly and efficiently. However, unlike an industrial or...
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