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How to be a Scientific Social Media King/Queen!

You have a nicely baked research project, all set and ready to tempt the world. Then comes an afterthought. It needs some spice, decoration, and even more. As a young or mature scientist your work is often...
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The Difference Between an Image, Flow, Time-lapse and Cell-sorting Cytometer

Ah, cell counting — it’s the oldest trick in the book! Well, not really, but people have been developing methods for counting cells since the late 1800s. It has been around for a while. But what different...
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Laboratory Animal Ethics: The Three Rs

Animal models have helped make enormous discoveries and breakthroughs in the last few decades. From Pasteur’s use of sheep to test the ‘Germ Theory’, Pavlov’s classic conditioning experiments in dogs,...
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Article

Cloning Large or Complex DNA Fragments

Sometimes you know a project is going to be a pain before you even start it. For me, that is whenever I need to clone large (> 3 kb) or complex (e.g., a sequence with repeats) DNA fragments. Long...
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Drift in Measurements with Analytical Balances

Pharmaceutical laboratories and bioscience research institutes make extensive use of analytical balances that are highly sensitive. These analytical balances are greatly affected by their environment and also...
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An Introduction to MassTag PCR

New ways to perform PCR emerge all the time. This speaks for the speed of technological advances, and reflects the ongoing need to keep up with fast-moving research. We all know that PCR’s main purpose is...
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How I Learned to Love Science All Over Again

A lot of grad students have probably seen this blog post on the development of science and had a good laugh about it. Long story short, if you imagine that all of human knowledge is a circle, the specialized...
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Extracting Better Ubiquitin Data from Your Samples: Beyond the Cellular Skip

The ubiquitin-proteasome system was discovered at the start of the 1980s, and people have been studying it ever since. Initially, researchers thought that tagging a protein with ubiquitin was the cell’s...
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Hierarchical or Boolean Gating: Which One to Choose?

A flow cytometer collects the events you are interested in, and also ‘sees’ every event that goes through. This includes debris and even bits in your buffers. As cytometrists, we gate our cells to exclude...
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Advocacy for Scientists – Why and How

Advocating for the research that scientists perform is important.  Your advocacy helps politicians and the general public understand why funding is needed. In fact, many funding agencies require a disease to...
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